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Patent Number: |
6716450 |
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Case ID: |
0 |
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Patent Title:
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Enhancing protein activity through nanoencapsulation
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Status: |
ACTIVE |
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Status Date: |
12/1/2004 1:59:25 PM |
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Issue Date:
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4/6/2004 |
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Filed Date:
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5/17/2001 |
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Serial #:
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859260 |
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Assignee Name:
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The United States of America as represented by the Secretary of the Army (Washington, DC)
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Inventor(s):
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Yin, Ray ,
Cheng, Tu-Chen ,
Durst, H. Dupont ,
Qin, Dujie |
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Lab Name: |
RDEC Edgewood Chemical Biological Center |
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Location: |
AMSRD-ECB-APB
Aberdeen Proving Ground, MD 21010-5424 |
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Contact: |
Contact Lab About This Patent |
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FIELD OF THE INVENTION
This invention relates generally to polymer nanoencapsulation and, more
particularly, to nanocapsule carriers, and a method of using these
carriers to enhance and stabilize enzyme reactivity or the reactivity of
other encapsulated bioactive molecules.
BACKGROUND OF THE INVENTION
This invention deals with nanoencapsulation and nanocapsules, and a method
of using these encapsulation systems to enhance protein/enzyme reactivity.
This invention also deals with using polymer nanocapsules to protect
proteins and enzymes from inactivation in a variety of harsh environments
such as extreme temperatures and pH. In addition, this invention also
provides a method for using nanoencapsulation to enhance protein/enzyme
reactivity in organic solvents. Finally, the present invention also
provides a method of using nanocapsules as controlled-release agents or
carriers for drug, protein, and vaccine del . . . . More |
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Nanocapsules useful for encapsulating bioactive molecules such as proteins
are disclosed. These nanocapsules are comprised of branched or
hyperbranched polymers and copolymers and have a core-shell structure
forming a pocket volume appropriate for complexing and retaining enzymes
and other bioactive molecules. The nanoencapsulated bioactive molecule is
stable in extreme temperatures and pH, soluble in aqueous or organic
solvents, and can be lyophilized to a dry powder for long-term storage
without loss of enzyme activity.
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What is claimed is:
1. A nanoencapsulated biomolecule, consisting essentially of:
(a) a nanocapsule having a core-shell structure, wherein said nanocapsule
consists essentially of randomly branched hydrophilic polymer having
hydrophobic chain-end moieties; and
(b) a bioactive molecule encapsulated within said nanocapsule.
2. The nanoencapsulated biomolecule of claim 1, wherein said nanocapsules
have a core-shell structure wherein the location of said chain-end
moieties in said structure will vary depending upon environmental
conditions.
3. The nanoencapsulated biomolecule of claim 1, wherein said hydrophilic
polymer comprises randomly branched polyoxazoline polymer.
4. The nanoencapsulated biomolecule of claim 3, wherein said polyoxazoline
polymer comprises polyethyloxazoline polymer.
5. The nanoencapsulated biomolecule of claim 4, wherein said
polyethyloxazoline polymer comprises poly (2-et . . . . More |
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